Protocols

Current Protocols

Virus Infections

• Baculovirus Methods
• Preparing Sf9 Lysate
• SV40 infection

Biochemical Assays

• E2F EMSA
• In Vitro DNA Replication
• Oligomerization Assay - Native Gel
• Single turnover assays
• Ssa1p purification
• TAg purification

Cell Culture

• Medium Cheat Sheet (Leigh Kridle)
• BSC40
• HeLa extract
• MEF
• REF52
• Protein Lysates/RNA (not Sf9)
• Sf9

• Anchorage Independent Assay
• In Vivo DNA replication
• Plaque Assay
• Transformation Assay

DNA

• Glycerol Stocks
• Interpreting Sequence Data
• Mini Preps
• Midi Preps
• Transform DH5-alpha cells

Digital Camera

• Digital Camera Use for Dummies